Strategic use of gene transfer technique for generation of marker-free and site-directed alien gene integration in plant genetic engineering of crop plants for improvement.

Strategic use of specially designed molecular construct for gene transfer will ensure generation of marker-free transgenic rice plants with high expression of target gene(s). This technical advantage can be realized from any plant system. The technical finesse of the molecular strategy to achieve such a gain forms the hallmark of this technology.

Biosafe and free from most of the major controversies that are raised in dealing with genetically modified crop plants by consumers and environmentalists.

This is a straight forward and practically achievable strategy for gene transfer method.